GRE-Luc Reporter Cell Line (293T)
Nuclear hormone receptors (NHRs) are a superfamily of transcription factors that function as powerful metabolic regulators to control a variety of systemic processes in physiology. They also play key roles in the pathophysiology of many major disease states, such as diabetes, obesity, inflammation, atherosclerosis and heart failure. To date, this superfamily has provided a rich source of drug design targets and it is continuing to be one of the hottest areas for pharmaceutical research. Glucocorticoids (GCs) are steroid hormones that allow us to cope with environmental and physiological stresses. Although GCs are widely used as anti-inflammatory agents in several clinical areas, long-term use of those steroids causes problems instead. Full achievement of the clinical potential of GCs necessitates limiting the side effects of these drugs. The GRE-Luc reporter cell line (293T) was created by stable co-transfection of 293T cells with a GRE-Luc (firefly) reporter plasmid and a puromycine expression plasmid followed by puromycin selection.