Mouse Peroxisome proliferator-activated receptor gamma ( PPAR gamma, Nr1c3) stable cells (Inducible)
Nuclear hormone receptors (NHRs) are a superfamily of transcription factors that function as powerful metabolic regulators to control a variety of systemic processes in physiology. They also play key roles in the pathophysiology of many major disease states, such as diabetes, obesity, inflammation, atherosclerosis and heart failure. To date, this superfamily has provided a rich source of drug design targets and it is continuing to be one of the hottest areas for pharmaceutical researches.
We provide a comprehensive set of NHR expressing cell lines to the research community. These high-quality, flag-and/or HA-tagged NHR-expressing cell lines aim to facilitate further biochemical and molecular studies of their functions and hence encourage new strategies for drug design.
The mouse PPAR gamma stable cell line was created by stable co-transfection of HEK293 cells with two plasmids expressing the bacterial Tet repressor (TetR) and the mouse PPAR gamma protein with both HA and FLAG tags, respectively. Since the mouse PPAR gamma gene is driven by a promoter containing two tetracycline operator (TeTO2) sites, its expression can be induced by doxycycline.