Irradiated 3T3-msCD40L Feeder Cells
Irradiated 3T3-msCD40L Feeder Cells
Catalog # C6003
For in vitro use only
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Alternative name |
3T3-msCD40L, 3T3-CD40L treated by irradiation |
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Description |
3T3-CD40L is a mouse fibroblast cell line that secretes mouse CD40L. 3T3-CD40L cells can be co-cultured with B cells to aid B cell expansion. Irradiated 3T3-msCD40L cells are treated by irradiation, and ready for feeding B cell culture. |
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Cell Type |
Mouse 3T3 cell line expression CD40L |
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Size |
1 vials (for 2 96-well plates) |
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Organism |
Mouse |
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Biosafety Level |
2 |
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Subculturing |
Adherent Cells, like fibroblast cells |
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Maintenance Tips |
Irradiated 3T3-CD40L cells are used for feeding B cell for once, and cannot be maintained for long term. |
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Propagation Medium |
DMEM with 10% FBS, 1% L-glutamine, and 0.1% gentamicin. |
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Reference |
J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013) |
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Note |
For laboratory research only. Not for clinical applications. |
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