Human B Cell Culture and Expansion Kit
Human B Cell Culture and Expansion Kit
Catalog # K1001
For in vitro use only
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Alternative name |
B Cell Expansion Kit |
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Description and Background |
Human B cell culture and expansion kit is for activation and expansion of human B cells. Human monoclonal antibodies isolated for specific antibodies are widely used in immunity studies, historical studies and therapeutic development. Memory B cells derived from peripheral blood will produce high concentration of IgG in the supernatant under the stimulation of CD40L with other growth factors. The B Cell Expansion Kit contains special treated feeder cells, expressing CD40L in activated form as well as human cell factors A and B potently inducing antibody-secret. The monoclonal antibody screening can be completed within 2 weeks with the help of the kit. |
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Components |
Special treated feeder cells, 7 x 106 Human cell factor A solution, premium grade Human cell factor B solution, premium grade |
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Biosafety Level |
2 |
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Reference |
J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013) |
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Note |
For laboratory research only. Not for clinical applications. For technical questions, please email us at info@humimm.com |
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