Anti-human CD3 [OKT-3] antibody
Clone: OKT-3 Catalog#: A10001
Description: The OKT-3 monoclonal antibody reacts with human CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The OKT-3 antibody has immunosuppressive properties in vivo and has been shown to effectively treat renal, heart and liver allograft rejection. It indirectly plays an important role in immunomodulation whereas the CD3 antibody may be used in in vitro Treg assays to generate effector Tcells.
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Isotype: |
Mouse IgG2a, κ |
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Species reactivity: |
Human |
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Production: |
Tissue culture |
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Purification: |
ProteinG purified |
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Recommended Isotype Control: |
Mouse IgG2a Isotype control |
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Applications: |
in vivo T cell depletion in vitro T cell stimulation/activation Immunofluorescence Flow cytometry Western blot |
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Immunogen: |
Human PBMCs were used as immunogen. |
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Storage: |
Store at +4°C short term (1-2 weeks). Store at -20°C long term. |
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Formulation: |
PBS, pH 7.4, Filter sterilized. Endotoxin level tests less than 0.06 EU/ug. |
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Clonality: |
Monoclonal |