
Anti-mouse CD25 (IL-2Rα) [7D4]
Clone: 7D4 Catalog#: A10018
Description: The 7D4 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The 7D4 antibody recognizes a different epitope on CD25 than the PC-61.5.3 clone.
Isotype: |
Rat IgM |
Species reactivity: |
Mouse |
Production: |
Tissue culture |
Purification: |
T-Gel |
Recommended Isotype Control: |
Rat IgM control |
Applications: |
in vivo regulatory T cell inactivation |
Immunogen: |
IL-2-dependent BALB/c mouse helper T-cell clone HT-2 |
Storage: |
Store at +4°C short term (1-2 weeks). Store at -20°C long term. |
Formulation: |
PBS, pH 7.4, Filter sterilized. Endotoxin level tests less than 0.06 EU/ug. |
Clonality: |
Monoclonal |