Cas9 Stable Cell Line for CRISPR (293T, inducible)

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Cas9 Stable Cell Line for CRISPR (293T, inducible)


Catalog # C0008


For in vitro use only



The recombinant, flag-tagged Cas9 (humanized S. pyogenes Cas9) is inducibly expressed in 293T in a tetracycline-dependent (0.1-1ug/mL) manner. This cell line is also G418 and puromycin (1ug/mL) resistant.


CRISPR, the Clustered Regularly Interspaced Short Palindromic Repeats, is currently the most commonly used technology for genome editing. There are two distinct components in this system: a guide RNA and an RNA-guided DNA nuclease, Cas9 (CRISPR associated protein 9). When these two components are expressed together in the same cells, the genomic sequence targeted by the gRNA will be edited (modified or permanently disrupted).

       Given the immediate availability of mouse and human lentiviral CRISPR gRNA libraries, CRISPR technology has tremendously benefitted the scientific community in both academia and industry. Humimmu is now providing tetracycline inducible Cas9 expressing cell lines of a variety of tissue origin to further facilitate high-throughput, CRISPR based genome-wide screening.

Host Cell

293T cells 


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